How is micropropagation performed? Which phenomena occur during telophase stage of mitosis in an animal cell? [3 + 2]
WBBSE
Class X
Life Science
02 (a) Cell Division and Cell Cycle
02 (b) Reproduction
05 Mark
2025
Micropropagation is rapid vegetative multiplication using tissue culture technique.​
It is performed in steps:
- Small living tissue sample (explant) taken from branch tip and sterilized.
- Placed on sterile nutrient agar medium with auxins/cytokinins in test tube.
- Forms callus/embryoids → plantlets regenerate.
- Plantlets hardened in soil under controlled environment. Used for orchids, gladiolus etc.
Telophase completes mitosis with nuclear reformation.
Daughter chromosomes reach poles and uncoil; nuclear envelope reforms around each; nucleolus reappears; cytokinesis begins by furrowing, forming two daughter cells.
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