Take two healthy potted plants, A and B of nearly the same size.
- Keep them in darkness for three days. (Destarch the plant)
- Place a watch glass containing potassium hydroxide by the side of potted plant A but not in potted plant B.
- Cover both the plants with separate bell jars and seal the bottom of the jars with Vaseline.
- Keep both the plants in sunlight for two hours.
- Pluck one leaf each from both the plants and test for the presence of starch with iodine solution.
- Observation: The leaf of the potted plant A with KOH did not turn blue-black, indicating that starch was not present. The leaf of the potted plant B turns blue.
- Conclusion: KOH absorbs CO₂ so photosynthesis did not occur in potted plant A.